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Suspension cell transfection efficiency monitoring


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#1 rahccoon

rahccoon

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Posted 22 November 2010 - 11:10 PM

Hello to all.
I wouldl like to monitor transfection efficiency of suspension cells with siRNA-lableed with flurorescent dye. Is there any way to estimate transfection efficiency besides using flowcytometer? For adherent cells, I could use fluorescent microscope but it may not be feasible for suspension cells. I would like to know if there is a way to estimate transfection efficiency of suspension cells using fluorescent microscope. Thank you for any suggestion!




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