HEK001 Cell Line and Papillomavirus
Posted 22 November 2010 - 05:08 PM
CAT # CRL-2404
Cells contain human papilloma viral DNA sequences.
(Papillomavirus 16 (HPV-16) E6/E7 transformed
ATCC has limited information on the cell line. From what I have found on line is min info.
What I am looking for is information on this cell line that states that the HEK001 cells can not generate a functional papillomavirus.
Any help on this would be great. I need to submit to my director a source that states that the cells "can not generate a functional papillomavirus"
Thank you for any help.
Posted 23 November 2010 - 02:11 PM
Posted 24 November 2010 - 03:00 PM
I did go through the paper.
The papillomavirus 16 E6 and E7 produces an immortal keratinocyte.
I imagine the biosafety level 2 comes from the fact that the cells produce some viral proteins, but not a functional virus.
Is this correct logic?
Thanks for the help.
Posted 24 November 2010 - 03:31 PM
Human epidermal T cells were transfected with a human papillomavirus 16 E6 and E7 plasmid in order to immortalize the the keratinocyte cells. They then named the cell line HEK001.
The only region they use of the viral genome is the E6 and E7 regions.
These are associated with "DISRUPTION OF CELL GROWTH CYCLES" only, hence, no generation of virus.
Edited by scs, 24 November 2010 - 03:32 PM.