Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

HEK001 Cell Line and Papillomavirus


  • Please log in to reply
4 replies to this topic

#1 scs

scs

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 22 November 2010 - 05:08 PM

RECEIVED HEK001 CELL LINE FROM ATCC
CAT # CRL-2404

http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2404&Template=cellBiology

Cells contain human papilloma viral DNA sequences.

(Papillomavirus 16 (HPV-16) E6/E7 transformed

ATCC has limited information on the cell line. From what I have found on line is min info.

What I am looking for is information on this cell line that states that the HEK001 cells can not generate a functional papillomavirus.

Any help on this would be great. I need to submit to my director a source that states that the cells "can not generate a functional papillomavirus"

Thank you for any help.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,833 posts
415
Excellent

Posted 23 November 2010 - 02:11 PM

Read the paper linked at the bottom of the ATCC page - it will tell you how the line was generated.

#3 scs

scs

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 24 November 2010 - 03:00 PM

Thanks for the reply.

I did go through the paper.

The papillomavirus 16 E6 and E7 produces an immortal keratinocyte.

I imagine the biosafety level 2 comes from the fact that the cells produce some viral proteins, but not a functional virus.

Is this correct logic?

Thanks for the help.

#4 scs

scs

    member

  • Active Members
  • Pip
  • 22 posts
0
Neutral

Posted 24 November 2010 - 03:31 PM

Here is some more info I found on the virus itself:

http://www.microbiologybytes.com/virology/Papillomaviruses.html

Human epidermal T cells were transfected with a human papillomavirus 16 E6 and E7 plasmid in order to immortalize the the keratinocyte cells. They then named the cell line HEK001.

The only region they use of the viral genome is the E6 and E7 regions.

These are associated with "DISRUPTION OF CELL GROWTH CYCLES" only, hence, no generation of virus.

Thanks

Attached Thumbnails

  • papova5.jpg

Edited by scs, 24 November 2010 - 03:32 PM.


#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,833 posts
415
Excellent

Posted 25 November 2010 - 04:09 PM

Correct.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.