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SoluBL21 strain

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#1 James W

James W


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Posted 22 November 2010 - 03:30 PM

Has anyone tried this new E. coli strain that is supposedly better than regular BL21 at making soluble protein?

For all I know they are regular BL21 cells and we are just given a different protocol:

Grow in minimal M9 media (basically salt water + .3% glycerol for a carbon source) instead of LB
Grow bacteria at RT instead of 37 degrees
Induce O/N at RT instead of 37 ~3hrs

So far it hasn't worked out too well; the cells hardly grow in this M9 media.

Edited by James W, 22 November 2010 - 03:31 PM.

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