resolving and stucking gel solutions
Posted 21 November 2010 - 02:41 PM
Im used to prepare the resolving gel by adding 1.5M Tris-Hcl pH 8.8 to other ingredients of SDS,APS; acrylamide, temed and water for the stucking gel, i used 0.5M Tris-Hcl pH 6.8. However, in my new lab, they are using 1M tris-HCl ph 8.8 for the resolving and 1M tris ph6.8 for the stucking!!
For me, its not very common, so, i follow this method, and it seems working, but im wondering if this could affect in some how my gel is good...
thanks for advice
Posted 21 November 2010 - 04:25 PM
Posted 22 November 2010 - 01:38 AM