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Distinguishing antibodies by their light-chain type


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#1 Chelo

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Posted 20 November 2010 - 10:21 AM

Hello dear colleagues,

Is there any easy way to separate antibodies (particularly, IgG) based on light-chain typing (lambda or kappa)?
Thanks a lot!

#2 BioMiha

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Posted 20 November 2010 - 12:42 PM

In an immunoassay using kappa or lambda specific secondary antibodies.

#3 sgt4boston

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Posted 22 November 2010 - 03:44 AM

I think you mean purify not quantitate? I believe you would first isolate all the IgG then run a column against the specific light chain you are intersted in thus your product would be IgG kappa or IgG lambda.




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