Posted 20 November 2010 - 04:32 AM
I made a SDS-PAGE gel to run my protein samples. When I prepare the seperating gel and overlay the 70% ethanol, the gel was in a good condition. Then I made the stacking gel and placed the comb. After 30 minutes I found some bubbles(~1mm dia) in the middle of the seperating gel.
Could anyone advice me in this regard. How can I get rid of this issue in future?
Thanks in advance!
Posted 20 November 2010 - 06:08 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 20 November 2010 - 06:27 AM
Did you degas the solutions?
Usually we dont degas the solutions. Could you tell me how to degass the solutions.........
Posted 20 November 2010 - 10:24 AM
Posted 22 November 2010 - 12:34 PM
genius does what it must
i do what i get paid to do
Posted 22 November 2010 - 05:28 PM
this could also happen if the gel is poured with cold solutions. trapped gasses will be released and form bubbles. if you allow the solutions to reach room temperature before pouring then degassing will be unnecessary.
Dear Biomiha and mdfenko,
Thanks a lot for your valuable advices!