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DMSO


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#1 samita

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Posted 20 November 2010 - 02:35 AM

In the site directed mutagenesis, we used DMSO.....in the PCR reaction...
what is the purpose of of DMSO in the PCR.
regards

#2 nightingale

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Posted 20 November 2010 - 03:13 AM

In the site directed mutagenesis, we used DMSO.....in the PCR reaction...
what is the purpose of of DMSO in the PCR.
regards


DMSO may enhance PCR, by eliminating non specific amplification or altering the thermal activity of the polymerase or that it may improve the annealing efficiencies of primers by destabilizing secondary structures within the template.

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