Posted 28 March 2002 - 07:18 AM
Mammalian cell transfection conditions must be determined empirically. You've tried electroporation. Try the chemical method-CaPO4 precip. If this does not work and your lab can afford it Life Technologies offers an optimization kit with several of their cationic lipids. Qiagen has an interesting technology with Superfect which is a dendritic polymer. They also have an optimization kit. Often transfection condition can vary from lab to lab with the same cell line. The growth conditions may vary slightly, the passage number may be an issue or the transfection gods may be angry. One just doesn't know. Good luck.