7 replies to this topic

[cellartist]

I am an artist wanting to depict the Tuberculosis bacterium wall with absolute accuracy.

Could anyone please let me know where might I find more information on
the actual physical appearance of:

Peptidoglycan
Mycolic acid
Dimycolate / Cord Factor
Wax-D

I am very grateful for any information. At this point I am not sure if the physical appearance has been determined. If it has not been I would appreciate any opinions on what they should look like based on function.


Thanks!

[bob1]

Your best bet will to be to go to a site like expasy.org or NCBI and search for the proteins there and see what comes up.  I would be surprised if some of them have not been rendered into 3D shapes.

[josse]

use google images..

Or like bob1 said: use expasy..

But how detailed do you need it?

I might be helpfull if you got hold of some picture made of the bacterium with a good microscope?

But are you planning on really drawing a structure of the cell with all the components in it?

It might also be helpfull if you check a general microbiology textbook like brooks to start with.

[cellartist]

Thanks so much for the links!  They are fantastic!

Regarding how detailed I want to get- as detailed as science has discovered and as realistic as possible.
I want it to be like the viewer was small enough to go inside the bacterium.

[pito]

a website like http://molvis.sdsc.e...du/fgij/  might be helpfull too.
(check http://proteopedia.o...ex.php/PDB_code for pdb codes)

Its not really for micro-organisms (not for a shematic drawing), but for more for proteins etc.. But it can be helpfull for you to get an idea and also some of the structures of the wall of a bacteria is based on proteins you find on that website.

and check http://www.nsrrc.org...2010/p60-63.pdf
To give you a more practical idea about what I mean.
(you can paint or draw a few of those proteins structes etc... because in most drawings they never show these and draw a cell wall as a "circle" but this not correct...

[cellartist]

Oh that is fantastic! Thank you so much!

[Majid]

I try to express protein in Sf9 cells.I'm using serum free medium from Novagen, in T-75 flasks. The cells are growing slowly and less than 10% are adhering. They accumulate in center of flask. What can I do to obtain a monolayer cell culture?
Thanks
Majid

[pito]

Majid, on 07 May 2011 - 10:50 PM, said:

I try to express protein in Sf9 cells.I'm using serum free medium from Novagen, in T-75 flasks. The cells are growing slowly and less than 10% are adhering. They accumulate in center of flask. What can I do to obtain a monolayer cell culture?
Thanks
Majid

I think its best you make a new post (topic) to try to get an answer to your question.