Where can I find info on the physical appearance of TB cell wall structures?
Posted 18 November 2010 - 09:09 PM
Could anyone please let me know where might I find more information on
the actual physical appearance of:
Dimycolate / Cord Factor
I am very grateful for any information. At this point I am not sure if the physical appearance has been determined. If it has not been I would appreciate any opinions on what they should look like based on function.
Posted 22 November 2010 - 04:23 AM
Or like bob1 said: use expasy..
But how detailed do you need it?
I might be helpfull if you got hold of some picture made of the bacterium with a good microscope?
But are you planning on really drawing a structure of the cell with all the components in it?
It might also be helpfull if you check a general microbiology textbook like brooks to start with.
Posted 23 November 2010 - 11:53 AM
Regarding how detailed I want to get- as detailed as science has discovered and as realistic as possible.
I want it to be like the viewer was small enough to go inside the bacterium.
Posted 28 November 2010 - 11:04 AM
(check http://proteopedia.o...ex.php/PDB_code for pdb codes)
Its not really for micro-organisms (not for a shematic drawing), but for more for proteins etc.. But it can be helpfull for you to get an idea and also some of the structures of the wall of a bacteria is based on proteins you find on that website.
and check http://www.nsrrc.org...2010/p60-63.pdf
To give you a more practical idea about what I mean.
(you can paint or draw a few of those proteins structes etc... because in most drawings they never show these and draw a cell wall as a "circle" but this not correct...
Posted 07 May 2011 - 10:50 PM
Posted 08 May 2011 - 01:40 AM
I try to express protein in Sf9 cells.I'm using serum free medium from Novagen, in T-75 flasks. The cells are growing slowly and less than 10% are adhering. They accumulate in center of flask. What can I do to obtain a monolayer cell culture?
I think its best you make a new post (topic) to try to get an answer to your question.