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How to convert ng/ul to ug/ul


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#1 Biochem_newbie

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Posted 18 November 2010 - 02:50 PM

Hi there

I know it seems like such an easy concept, but if someone could give a helping hand in helping me understand the concept, I would greatly appreciate it!

So from this website, I gather that if I have a starting concentration of 500 ng/ul and my desired concentration is 0.1 ug/ul with a final volume of 100 ul, the amout of sample to dilution water would be 20 mL solution and 80 mL water.

http://www.functiona..._mass_input.php

Could someone explain this to me?
Thank you so much for your time!

Edited by Biochem_newbie, 18 November 2010 - 02:51 PM.


#2 bob1

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Posted 18 November 2010 - 04:42 PM

1 ng/ul = 0.001 ug/ul

Use V1 x C1 = V2 x C2 (where V = volume and C= concentration) Keep the units the same (i.e. if using ul, keep it all in ul).

#3 BioMiha

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Posted 18 November 2010 - 10:16 PM

0.1 ug/ml is 100 ng/ml. 500 ng/ml is 5 x 100 ng/ml meaning you have to dilute 5-fold => 20 ul + 80 ul

#4 nightingale

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Posted 19 November 2010 - 01:42 AM

let just show you an easy way to convert between units :
ng to ug ...
convert the nanogram to grams, then the grams to micrograms ...

for example 5 ng = ?? ug

5 ng x 1 g = 5 x 10 ^ -9 g
10 ^ 9 ng

(5 x 10 ^ -9 )g x (10 ^ 6) ug = 5 x 10 ^ -3 ug
1 g

so 5 ng = 0.005 ug

without the need to memorize anything as most students do ...

hope i have simplified it ! :)

" The more you learn, the more you realize how little you know ... "




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