Viability of intracellular bacteria
Posted 18 November 2010 - 04:16 AM
From my timelapse imaging, I always observe my bacteria trap in late endosomal compartments, even after overnight. Although the bacteria look fairly intact from the morphology, I would like to know if the bacteria are still viable.
Do you know if there is any way I can check it?
I came across the Live/Dead Baclight staining kit from Molecular Probes, but not sure if this kit is going to stain the intracellular bacteria specifically, or it will stain up my cells simultaneously.
I also thought of lysing the cells to release the intracellular bacteria and plate them on agar plates. However, this approach will only work if the gentamicin work at 100% efficiency to kill of the extracellular bacteria. Normally, I still get 1 or 2 colonies on my agar plate when I plate the medium containing gentamicin at the end of the experiment.
Any idea from all the genius out there?????
Many thanks in advance.
Posted 22 November 2010 - 04:11 AM
Edited by microRNAboy, 22 November 2010 - 04:11 AM.