serum separation: transparent gel prevents serum collection!
Posted 17 November 2010 - 11:59 AM
And what happens if I centrifuge before the clot forms, do I get also the coagulation factors together with the serum? (I am using plain glass tubes, with no anticoagulant and I am collecting 3 ml of blood).
Thanks for any help ypu can give me, as you see I am a very beginner in this field!
Posted 18 November 2010 - 03:41 AM
I am making an assumption that the protein is stable frozen and does not change upon thawing. If this is the case then you will have to explore diluting the sample in some type of buffer to preserve it. There are several additives and buffers on the market.
If you are running an elisa then what are you using for your standard or calibrators and what matrix is it in? Your samples should have about the same stability (with azide/thimerosal/etc).
Posted 18 November 2010 - 03:44 AM