Posted 17 November 2010 - 04:20 AM
i did WB on samples from brain tissue, that was in -20°C since 2 or 3 months, and some 1 week.
i saw different profile on my filter after w.b. the signal is lower in the samples kept 3months in -20°C. morever, there are some bands at the top of the filter. but may be this is due to aspecifics because of secondary antibody..
if u have ideas about this topic, thanks for your interaction
Posted 17 November 2010 - 08:46 AM
genius does what it must
i do what i get paid to do
Posted 17 November 2010 - 01:52 PM
Posted 17 November 2010 - 02:04 PM
smears in below!! so, i think its my samples which are degradated.