protease inhibitors and bradford
Posted 17 November 2010 - 04:11 AM
I ve qantified protein extract of brain tissue, solubilized in NP40buffer with protease inhibitors, phosphatase inhibitors and sodium floride.
so, im wondering if this could interfere with bradford and affect the quantification (BSA). also i use blank in which i put the buffer NP40 with that inhibitors as well.
thanks for advice
Posted 17 November 2010 - 08:40 AM
but, the readings must remain within the range of the standards (you can also add the blanking buffer to the standard curve).
Edited by mdfenko, 17 November 2010 - 08:42 AM.
genius does what it must
i do what i get paid to do
Posted 17 November 2010 - 02:18 PM