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Is it possible to compare data between real-time PCR plates

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#1 MMQ1



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Posted 16 November 2010 - 06:27 AM


My question is regarding whether it is possible to compare expression of genes between realtime PCR plates if I include the same calibrator sample in each plate which I use for the deltadelta Ct calculation each time. In my experiment I have samples from 5 different patients but due to the number of treatments I can only fit one patient per plate. So I just wanted to double check that it is possible to compare data collected from different Qrt-PCR runs.

Thanks in advance for your help.

#2 pcrman



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Posted 16 November 2010 - 08:02 AM

Yes, it is possible. If you are using the ABI program, go to New Analysis -> Add Plates.

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