3 replies to this topic

[damon]

Does anybody know the unit conversion between u/ml and ug/ml?Well,this unit is used to measure the concentration of TNF-α.
To be honest, I doubt whether they can convert.So just tell me the truth.

[almost a doctor]

damon, on 15 November 2010 - 07:41 PM, said:

Does anybody know the unit conversion between u/ml and ug/ml?Well,this unit is used to measure the concentration of TNF-α.
To be honest, I doubt whether they can convert.So just tell me the truth.

You need to know how many TNF-a units are per ug, and then is pretty straight forward.  

say you have x u/ug and  y u/ml,    y/x will give you ug/ml.

[rhombus]

damon, on 15 November 2010 - 07:41 PM, said:

Does anybody know the unit conversion between u/ml and ug/ml?Well,this unit is used to measure the concentration of TNF-α.
To be honest, I doubt whether they can convert.So just tell me the truth.

With enzymes for example the "specific activity " will be given in units/mg on the vial. The vial is normally supplied with a predetermined volume of enzyme, for example 1ml. You then have to convert mg/ml into units/ml if required. This is a way of quantifying an exact amount of a product into an experiment, as the number of units between enzyme batches vary.

On your vial of TNF alpha there will be a total number of units/vial. Also it should indicate the number of units/mg of TNF. If you know the volume then it is a simple calculation to convert into units/ml.

i.e. Vial of TNF (1mg) = 50,000 units/mg
     Volume of liquid = 1ml

Therefore your stock solution = 1mg/ml  or 1ug/ul  or
                                50,000u/ml or 50u/ul

Does this help or is it too confusing still.

Kindest regards

Uncle Rhombus

[damon]

Guys,I really appreciate your reply.As a chinese ,especially has little talent in english, I will find it hard to understand what you are talking about sometimes.But your patience finally help me get the right answer.
Best regards,
Damon