More and more annoying questions
I need to get my protein concentration higher, it's currently only 500 ug/ml and it needs to be at least 1 mg/ml. I have used Millipore filter devices before, but somehow my protein didn't like it and I ended up losing pretty much 70% of the protein sample in the process. I'm now trying to use TCA, but keep reading some horror stories about it too... so I was wondering is precipitation the protein with TCA ok? And how much you normally manage to get out by using it (hopefully more than 70%). I'm a bit desperate, like always
And what is the best TCA protocol or the one which has at least worked for someone?
I plan to dissolve the protein in the end to 8M urea... so in theory it should hopefully dissolve.