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Agarose gel electrophresis


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#1 Bilal Malik

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Posted 15 November 2010 - 02:03 AM

Hello.... I have a question regarding the agrose gel electrophoresis.. Is there any way to differentiate betwen two bands having a few base pair differences i.e. 122bp and 119bp.. help

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  • 2re-extraction with Mini elute kit with enzyme in individual sample.gel 2010-10-30 15hr 33min.jpg


#2 BioMiha

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Posted 15 November 2010 - 03:14 AM

You could try resolving on a polyacrylamide gel. Like in the old days of sequencing. The problem with PAGE is that you cannot add EtdBr directly into the gel, so you have to incubate the gel in EtdBr after electrophoresis and the contrast is not quite the same. Otherwise it works.

#3 chandler

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Posted 19 November 2010 - 07:49 PM

I agree with upstairs, you can not distinguish 121bp and 119bp with agarose electrophoresis. Good luck.

#4 Bilal Malik

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Posted 21 November 2010 - 08:45 PM

10nx alot for your replies ... will try it with PAGE..lets see if it works.... 10nx again




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