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Electrophoresis Profil shifted up or down


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#1 keskispasse42

keskispasse42

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Posted 14 November 2010 - 11:16 PM

Hi,

I have used arbitralily primed pcr for bacteria typing. I few cases I have some trouble with particular strain. I AP-PCR profil shit down during electrophoresis. If I repteat the electrophoresis only I have the same profil but shifted down. This problem occure most often with primer ERIC2 but I have also the same problem with the primer 1. The excration was automatised et the amplification was realized on the same plate.
Electrophoresis was performed as following : TAE 1X, Agaraose invitrogen 1.5% with TAE 1x, 200V during 1h (cuve of 30 cm), buffer loadding home made (glycerol 50%, bromophenol blue at 1 mg/ml). This probleme was illustrated on the picture : Lanes 18, 19 and 21 show the same profil but the lane 18 was shifted down.

Thanks a lot for your suggestions !

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