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Collagen type III gel/fiber formation in vitro


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#1 Trancerules

Trancerules

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Posted 13 November 2010 - 06:37 PM

Dear all,

As you might know, there are protocols available for making collagen type I gels. I am currently using the one from BD Biosciences(add 10XPBS and water, and neutralizing using 1N NaOH). I have fine-tuned this protocol and can make collagen type I gels/fibers. However, I am now trying to make collagen type III gels. Being an EE student, I have limited knowledge in biochemistry, and do not know how to proceed from here. I havent not found any article that makes collagen III gels in a simple way (I mean something close to the current protocol of making type I gels). I know that the collagen molecules start polymerizing under neutral pH. So in principle, it seems like I should start with the same procedure as type I, right?. Any advice/suggestions are greatly appreciated. Please reply at your earliest convenience.

Thanks
Trancerules




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