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Is CNBr activated Sepharose good for binding IgG?


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3 replies to this topic

#1 KWY

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Posted 12 November 2010 - 11:10 AM

I want to purify a antigen, and i got its rabbit form antibody IgG
I dont have DMP/DSS and it will take few weeks to order and for its arrived
but i got some CNBr activated Sepharose , i know that it will attached to the amino group of the protein
will it be effective for binding IgG without losing the activity of IgG?

Thanks for any advice!!!

#2 mdfenko

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Posted 12 November 2010 - 01:16 PM

cnbr-sepharose will bind the antibody but its orientation will be random. because of this not all of the bound antibodies will be active.
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genius does what it must
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#3 KWY

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Posted 13 November 2010 - 08:00 AM

so do u suggest i should do cross linking with DSS/ DMP?

#4 mdfenko

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Posted 15 November 2010 - 08:17 AM

if you are referring to crosslinking the antibody to protein A (or G or A/G) agarose, then yes. this way all of the antibodies should be active for capture of your antigen.
talent does what it can
genius does what it must
i do what i get paid to do




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