Posted 12 November 2010 - 04:20 AM
I have attacged the picture and it seems to me G is there but peak is not visible...
or its deletion mutation from polymerase by itself?????
Posted 12 November 2010 - 06:20 AM
Posted 12 November 2010 - 07:06 AM
I have attached the new picture
WT = 5-GACCTAC-3
I used the reverse complementary sequence from the sequncing file to align with wild type...
Posted 12 November 2010 - 02:07 PM
I assume this is the problem you are worrying about -- although I still can't make any sense out of the top trace.
If I read things correctly, the answer is "don't worry, be happy."