2 replies to this topic

[Parichut]

Dear all

     I want to extract DNA from paraffin embbeding tissue which already stain with H&E. After staining, i have to select cell from tissue by manual technique. However, i don't know what is the buffer that use to pick up cell from tissue (Should have protease in buffer? ). What is chemical component of that buffer? How i get DNA from that tissue which have very low amount of cell by use phenol chloroform technique?
Note: My tissue have 4 um thick and have area 1x1 cm (liver section)

Thanks

[nightingale]

Hello Parichut,

have a look at this Protocol Online Archives
i remember discussing this here, before ...

hope you will find it beneficial

Best Wishes

[Parichut]

Thank you so much.