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DNA extraction from paraffin embbed tissue


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#1 Parichut

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Posted 11 November 2010 - 11:57 PM

Dear all

I want to extract DNA from paraffin embbeding tissue which already stain with H&E. After staining, i have to select cell from tissue by manual technique. However, i don't know what is the buffer that use to pick up cell from tissue (Should have protease in buffer? ). What is chemical component of that buffer? How i get DNA from that tissue which have very low amount of cell by use phenol chloroform technique?
Note: My tissue have 4 um thick and have area 1x1 cm (liver section)

Thanks

#2 nightingale

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Posted 12 November 2010 - 01:15 PM

Hello Parichut,

have a look at this Protocol Online Archives
i remember discussing this here, before ...

hope you will find it beneficial :)

Best Wishes

" The more you learn, the more you realize how little you know ... "

#3 Parichut

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Posted 13 November 2010 - 09:17 PM

Thank you so much. :lol:




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