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[Meta]

Hi,
I have an extract that has a bunch of different genome sizes (10kb - 3Mb). I'm trying to get at the genomes that are less than ~200kb and I'm thinking I can do this on a standard agarose gel (would rather avoid PFGE is possible).

Anyone know if I can get a separation of something like above 200kb and below 200kb on a gel? Any thoughts on what agarose (type and concentration) and voltage would work?

Also any insight of other ways to separate out genomes based on size would help too.

Thanks

[phage434]

This isn't going to work.  Agarose gels don't separate above about 20Kb or so, regardless of concentration.  Field inversion gels (FIGE) would do this job easily.