Im using the Tet inducible system in Haemohpilus influenzae (Gram-) to drive expression of a GFPmut3 reporter plasmid. We succesfully use it in broth culture with anhydrotetracyline as inducer at concentrations of 50-200ng/ml with good induction. I would like to do my experiment on a chocalate agar plate, but have not been able to find what concentrations of aTet that should be used. Has anyone used aTet in plates before? We are not sure if the aTet will be used up on the surface and thats it. So Im not sure if you can use aTet in plates like you can use IPTG.
anhydrotetracycline in agar plates?
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