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Purified lambda phages


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#1 tgiffon

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Posted 11 November 2010 - 04:44 PM

Goof afternoon,

I'm trying to purchase lambda phage particles (purified if possible) to use as a control for proteinase K digestion in a restriction analysis assay! I can readely find purified DNA from this bacteriophage but so far I have not find any place selling either live of purified viruses.
Would anybody know a company selling this??
Thanks for the help!
Cheers
Thierry

#2 phage434

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Posted 11 November 2010 - 06:11 PM

Carolina Biological sells inexpensive lambda phage cultures. Your best bet is likely to grow some up and sterile filter out the bacteria, leaving phage. Or, do a chloroform extraction, which is the normal way of making an active lysate.




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