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Maturation Cd11b+ cell culture

Started by Sbdt, Nov 11 2010 02:15 PM

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#1 Sbdt

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Posted 11 November 2010 - 02:15 PM

Hi,

I harvest cd11b+ monocytes from mice and I would like to differentiate them into macrophages. What differentiating factors could I use in the culture media? M-CSF-1? PMA? and which concentrations?
Thanks for your help,

Sbdt

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