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how to determine gel percentage?


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#1 SF_HK

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Posted 10 November 2010 - 04:57 PM

HI,

I have to detect cleaved caspase-3 (17kDa) using western. How much percentage gel should I be using and shall I try gradient gel?

thanks

#2 protolder

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Posted 10 November 2010 - 10:45 PM

Hola, to see a 17KD band isnīt necessary to make a gradient gel; with a 12% gel it will run near the botton and in a 15% around the half.Using any of the common colored marker you could follow the 14 kd band and 21 kd band. Good luck




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