1) Without lysing the platelets, would the platelet proteins just clump up on the top of a SDS-page gel and not correctly run since they are still contained in the cell fragment?
2) Without lysing the platelets and removing the cell debris, would not the platelets just collect at the bottom of a vial?
3) I believe the lab is wanting to test proteins within the thrombocytes and without lysing, the antibodies would not actively bind. Is this correct or is there something about the thrombocytes that I am overlooking.
Thanks for any help provided.
Edited by scs, 10 November 2010 - 04:03 PM.