cell culture media
Posted 10 November 2010 - 07:24 AM
Posted 10 November 2010 - 04:06 PM
- fatemeh likes this
Posted 11 November 2010 - 03:32 AM
I kept my DMEM media overnight at waterbath by mistake. Shall I discard that?
My motto is:-
"If in doubt then chuck it out".
A bottle of media is cheap....the experiments you might do with them can be very expensive. The idea with research is not to change standard operating procdures (SOP)and by using this media that's what you are doing. I have read papers where it has been reported that light senstive proteins in media can be affected by long periods of light exposure. Remember that media is usually stored in a cold room or upright fridge ...with the lights off. The media also comes in cardboard boxes which again protects it from light when delivered. I know of some researchers in my Institute that cover the media bottle in silver foil, again to protect it from the light.
Question: Is there a light in a CO2 Incubator ?
Sorry bob1, but on this ocassion I disagree with you advice.
Posted 11 November 2010 - 07:51 AM
It would be far easier to make a new bottle of media than to sit worrying about your experimental results (or potentially lack thereof)
In our lab, if a media bottle is accidentally left anywhere but in the fridge overnight, it's chucked and a new one made.