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lentivirus


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#1 vetticus3

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Posted 10 November 2010 - 01:42 AM

Hi,
Ok, so i've made lentivirus before, etc... but, i was wondering, has anyone used two different transfer vectors in the same virus production step?
ie, vsvg, gag/pol, rev all the same, but instead of just one shrna (or whatever), have 2 (but kept at the same concentration)?
does this sound completely stupid?

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#2 Functional Screens

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Posted 28 November 2010 - 01:13 PM

Of course you can do that, but why not generate the lentiviruses separately, titer them and then mix them with the equal titer?




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