We test our antibodies via Western Blot
We have some cell lines that yield very low concentrations of nuclear proteins such as A431
I came across some protocols that remove nucleic acids from the nuclear protein extract.
1) Is there any interference on SDS-page with the presence of nuclear acids in the nuclear extract?
2) Is there any reasons why I should remove nucleic acid from our nuclear extracts?
3) Is there any ideal protocol / set of reagents that really can maximize the amount of nuclear proteins that can be extracted from mammalian cell lines?
(A protocol where someone has really noticed significant gain compared to other protocols)
4) We dialyze our product at the end of the protocol, but I have read that this is not optimal. Is this true?
Thank you so much for the help.
Edited by scs, 09 November 2010 - 08:41 AM.