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Increasing hybridisation efficiency using Cytoclear


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#1 Tooney

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Posted 08 November 2010 - 07:32 PM

Hi
hybridisation with radiolabelled and non-radiolabelled probes on chromosomes embedded in cytoplasm is low due to the weak penetration of the probe during hybridisation. I have discovered that we can improve the efficiency of the hybridisation by washing the cell suspension in Procell Cytoclear before slides are processed for hybridisation. I highly recommend the method. Our vendor is Rainbow Scientific, but I am sure others distribute the product. Hope tjis tip helps others?

#2 Zagami Francesco

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Posted 03 April 2014 - 08:14 AM

Clear solution : dissolve 100 µg/ml pepsin in 0.01 N di HCl containing 0.1 % di Tween-20.
For specific application protocols ask to francesco_zagami@virgilio.it





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