DNA from ticks
Posted 08 November 2010 - 10:49 AM
What is the best method for extracting DNA from ticks ?
I heard of a heating method of ticks in a solution of ammonium.
Thanks in advance
Posted 08 November 2010 - 05:51 PM
Posted 08 November 2010 - 11:22 PM
For ticks I would try a Chelex method: you prepare 10% Chelex solution, squash the ticks, and incubate it for some hours at 65 °C, add Proteinase K, centrifuge, dilute and have ready to use tick DNA for standard PCR applications. Most commercial kits and standard DNA extraction procedures based on detergent, chloroform, EtOH will also work, which method to use depends on your budged and lab equipment.
If interested in blood, I would suggest a kit.
Posted 09 November 2010 - 10:46 AM
Edited by hobglobin, 09 November 2010 - 10:47 AM.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.