Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA from ticks


  • Please log in to reply
3 replies to this topic

#1 Lilia

Lilia

    member

  • Members
  • Pip
  • 1 posts
1
Neutral

Posted 08 November 2010 - 10:49 AM

Hello,
What is the best method for extracting DNA from ticks ?
I heard of a heating method of ticks in a solution of ammonium.
Thanks in advance
Lilia

#2 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
7
Neutral

Posted 08 November 2010 - 05:51 PM

Do you want the tick DNA or the the DNA from the blood the tick ate?
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 08 November 2010 - 11:22 PM

I have not heard about the ammonium method, but it might work as well.

For ticks I would try a Chelex method: you prepare 10% Chelex solution, squash the ticks, and incubate it for some hours at 65 C, add Proteinase K, centrifuge, dilute and have ready to use tick DNA for standard PCR applications. Most commercial kits and standard DNA extraction procedures based on detergent, chloroform, EtOH will also work, which method to use depends on your budged and lab equipment.

If interested in blood, I would suggest a kit.
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#4 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,546 posts
104
Excellent

Posted 09 November 2010 - 10:46 AM

A salting out protocol such as from Aljanabi & Martinez (1997): Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research 25: 4692–4693. should do the job for tick DNA. If you need the blood from the engorged females, I'd use a hollow needle/cannula from a syringe to get the blood without contamination with tick tissue and stuff and then continue with a kit (as suggested above).

Edited by hobglobin, 09 November 2010 - 10:47 AM.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

That is....if she posts at all.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.