Posted 08 November 2010 - 09:53 AM
Posted 08 November 2010 - 10:15 AM
Posted 08 November 2010 - 10:57 AM
Posted 08 November 2010 - 03:14 PM
ELISA is better for quantification. Protein of Interest is better purified by IP. For sure, thats not complete answer to your question. May you tell us a little bit more about goal of your experiment.
I agree with the above. I think western blotting is cheaper though and easier to do in theory.
However, western blotting is more finicky.. Its one of the techniques that requires a special touch if you are probing for something that is in low quantities or if you are using a not very reliable antibody.
ELISA are quicker from my experience and more reliable for quantifying.
You can quantify relative amounts using western blot though.