does anybody know what are the disadvantages of Western Blot?
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#2
ksturm
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Posted 08 November 2010 - 10:15 AM
Would be helpful to precise your question. Disadvantages of Western in comparison to what technique?
#3
evie
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Posted 08 November 2010 - 10:29 AM
ELISA and Immunoprecipitation.
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ksturm
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Posted 08 November 2010 - 10:57 AM
ELISA is better for quantification. Protein of Interest is better purified by IP. For sure, thats not complete answer to your question. May you tell us a little bit more about goal of your experiment.
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UBClabbie
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Posted 08 November 2010 - 03:14 PM
ksturm, on 08 November 2010 - 10:57 AM, said:
ELISA is better for quantification. Protein of Interest is better purified by IP. For sure, thats not complete answer to your question. May you tell us a little bit more about goal of your experiment.
I agree with the above. I think western blotting is cheaper though and easier to do in theory.
However, western blotting is more finicky.. Its one of the techniques that requires a special touch if you are probing for something that is in low quantities or if you are using a not very reliable antibody.
ELISA are quicker from my experience and more reliable for quantifying.
You can quantify relative amounts using western blot though.
