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western blot


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4 replies to this topic

#1 evie

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Posted 08 November 2010 - 09:53 AM

does anybody know what are the disadvantages of Western Blot?

#2 ksturm

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Posted 08 November 2010 - 10:15 AM

Would be helpful to precise your question. Disadvantages of Western in comparison to what technique?

#3 evie

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Posted 08 November 2010 - 10:29 AM

ELISA and Immunoprecipitation.

#4 ksturm

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Posted 08 November 2010 - 10:57 AM

ELISA is better for quantification. Protein of Interest is better purified by IP. For sure, thats not complete answer to your question. May you tell us a little bit more about goal of your experiment.

#5 UBClabbie

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Posted 08 November 2010 - 03:14 PM

ELISA is better for quantification. Protein of Interest is better purified by IP. For sure, thats not complete answer to your question. May you tell us a little bit more about goal of your experiment.


I agree with the above. I think western blotting is cheaper though and easier to do in theory.
However, western blotting is more finicky.. Its one of the techniques that requires a special touch if you are probing for something that is in low quantities or if you are using a not very reliable antibody.

ELISA are quicker from my experience and more reliable for quantifying.
You can quantify relative amounts using western blot though.




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