I am doing starvation experiment for nitrifying bacteria in wastewater and I want to know only the live bacteria for nitrobacter and nitrosospira for example. I am using T-RFLP. The normal PCR will amplify both the live and the dead bacteria DNA as I thank. Any suggestions
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Is it possible to know only the live bacteria using PCR for a certain genus?
Started by Abdelsalam Elawwad, Nov 06 2010 01:08 PM
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Posted 08 November 2010 - 01:57 AM
Abdelsalam Elawwad, on 06 November 2010 - 01:08 PM, said:
I am doing starvation experiment for nitrifying bacteria in wastewater and I want to know only the live bacteria for nitrobacter and nitrosospira for example. I am using T-RFLP. The normal PCR will amplify both the live and the dead bacteria DNA as I thank. Any suggestions
FACS sorting. Don't know if you have, but would work.
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