Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Concerned about sterile technique


  • Please log in to reply
9 replies to this topic

#1 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 329 posts
25
Excellent

Posted 06 November 2010 - 02:22 AM

HI all,

This is not exactly a rave/rant but my lab mates do not have a very good sterile technique. It often results in contamination of the samples they are working with.

The problem is I am new to the lab and their work is independent of the work I do. So, telling them that their sterile technique is not very good is none of my business (I am not their boss either), but it hurts to see someone answer the phone with gloves on and go back under the hood to finish off the work. Basically, I am looking for a good way to tell them/ make them realise their mistake. Something like a funny poster or any other ideas you may have.

Thanks for your help ....
:)

NEW!!!!  Living with Malaria on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#2 perneseblue

perneseblue

    Unlimited ligation works!

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 578 posts
16
Good

Posted 06 November 2010 - 04:54 AM

If you also use the hood, their lack of sterile technique will be your business. It is likely that the hood is no longer sterile. You can not work in there.

Still you could drop by and say, "hey guys, I got a few trick to reduce contamination problems with your cultures. I'll like to show them to you."
May your PCR products be long, your protocols short and your boss on holiday

#3 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 329 posts
25
Excellent

Posted 07 November 2010 - 10:17 PM

If you also use the hood, their lack of sterile technique will be your business. It is likely that the hood is no longer sterile. You can not work in there.


Well, I do not use the hood thats why its none of my business.

Still you could drop by and say, "hey guys, I got a few trick to reduce contamination problems with your cultures. I'll like to show them to you."


This is what I am not sure about. Would they take it well? It is blowing my own horn to say I'd like to show you how to do your work. Thats why I am looking for something... which could be taken in a jocular manner and the message is also sent through...
:)

NEW!!!!  Living with Malaria on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,709 posts
123
Excellent

Posted 08 November 2010 - 11:32 AM

the next time you see one of your labmates complain about a contaminated culture you can approach them and say that you learned some tricks to prevent contamination in the last lab in which you worked. then say that you'll share them if they are interested.
talent does what it can
genius does what it must
i do what i get paid to do

#5 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 329 posts
25
Excellent

Posted 08 November 2010 - 08:50 PM

the next time you see one of your labmates complain about a contaminated culture you can approach them and say that you learned some tricks to prevent contamination in the last lab in which you worked. then say that you'll share them if they are interested.


Guess thats the only way to do it. Thanks for your suggestions.... and if you do come across something which might help, kindly post it to me.

Thanks again! :)

NEW!!!!  Living with Malaria on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist


#6 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 08 November 2010 - 11:16 PM

answering the phone while working in a sterile bench is deadly sin #8. If you are working in a microbiological lab it is important to teach them lab safety. But this will be the duty of your boss to tell them.

What you can do was suggested in the previous posts; another thing which might work is having a list of dos and don'ts prepared that you hand over to the lab manager/boss because you just got it sent by a friend working somewhere else and if (s)he thinks it would be useful to email it around.
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#7 vetticus3

vetticus3

    Princess

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 144 posts
9
Neutral

Posted 10 November 2010 - 05:29 AM

Or you could simply tell them, in a nice way, that you shouldn't answer the phone with gloves on.

#8 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
7
Neutral

Posted 14 November 2010 - 10:14 PM

All of the above.

Be as nice as possible.

Learn from my mistake: I had a meeting with one of my bosses, because I let my professionalism drop and thoughtlessly commented on where I thought the source of the contamination originated. The woman I said this to was immediately offended.

Edited by lab rat, 14 November 2010 - 10:16 PM.

42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#9 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,267 posts
76
Excellent

Posted 15 November 2010 - 01:49 AM

I trick I sometimes used is not to tell them it , but to ask them it...

Ex.: if you see them do that, just ask them: oh, you are allowed to go away from the hood and enter again without using ethanol ? Strange, I have alwyas been told that I should not do that.. Good to know, I do not need to use ethanol all the time.. its saves me time...
Or something like: you can pick up the phone and continue back working because the ethanol in still "active" on the gloves and still killing everyhting?


Something like that.. just act like a newbie,someone who doenst know much, fresh from school...

(it might make you look a bit "stupid", but its better then starting troubles.. a lot of the people that have been working for ages with wrong techniques arent really friendly and are not prepared to listen to advice or whatever..., but if you ask it as a question: they can accuse you of "acting like their boss"...)

PS. I do hope they arent working with pathogens... if so: they are really doing a great job infecting everything.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#10 Ameya P

Ameya P

    Rervm Cognoscere Cavsas

  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 329 posts
25
Excellent

Posted 15 November 2010 - 04:10 AM

I do hope they arent working with pathogens... if so: they are really doing a great job infecting everything.


Luckily, its not a microbio lab they are working in. So, they are not infecting anyone around, except for the tissues they are working with.....

NEW!!!!  Living with Malaria on CoffeeTableScience!!!!

Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.