Does anyone know of any peptide markers for native PAGE ~4kDa? I found some low range markers but they seem to be for SDS PAGE.
0
3 replies to this topic
#2
mdfenko
-
- Active Members
-
- 2,288 posts
an elder
83
Excellent
Excellent
Posted 05 November 2010 - 12:01 PM
you can use the peptide marker kit from ge healthcare. resuspend in water or suitable buffer instead of the sds-urea loading buffer recommended in the instructions that come with them.
Edited by mdfenko, 05 November 2010 - 12:02 PM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
donny
-
- Active Members
-
- 73 posts
Enthusiast
3
Neutral
Neutral
Posted 07 November 2010 - 10:09 PM
Thanks for the suggestion. I saw that one too but I wasn't too sure if it would work for native PAGE since it was intended for SDS PAGE. I know some SDS-PAGE protein markers cannot be used for native PAGE, possibly due to dissociation of oligomers. Would such complications apply to the said peptide marker eg. difference in conformation?
#4
mdfenko
-
- Active Members
-
- 2,288 posts
an elder
83
Excellent
Excellent
Posted 08 November 2010 - 12:15 PM
i haven't seen any problem using them in a native gel. you can confirm that by running 2d (native/sds) page on the markers.
most sds-page markers can't be used in native page because they have already been denatured with sds.
most sds-page markers can't be used in native page because they have already been denatured with sds.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
