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peptide markers for native PAGE


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3 replies to this topic

#1 donny

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Posted 04 November 2010 - 08:55 PM

Does anyone know of any peptide markers for native PAGE ~4kDa? I found some low range markers but they seem to be for SDS PAGE.

#2 mdfenko

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Posted 05 November 2010 - 12:01 PM

you can use the peptide marker kit from ge healthcare. resuspend in water or suitable buffer instead of the sds-urea loading buffer recommended in the instructions that come with them.

Edited by mdfenko, 05 November 2010 - 12:02 PM.

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#3 donny

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Posted 07 November 2010 - 10:09 PM

Thanks for the suggestion. I saw that one too but I wasn't too sure if it would work for native PAGE since it was intended for SDS PAGE. I know some SDS-PAGE protein markers cannot be used for native PAGE, possibly due to dissociation of oligomers. Would such complications apply to the said peptide marker eg. difference in conformation?

#4 mdfenko

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Posted 08 November 2010 - 12:15 PM

i haven't seen any problem using them in a native gel. you can confirm that by running 2d (native/sds) page on the markers.

most sds-page markers can't be used in native page because they have already been denatured with sds.
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