How does the RBC lysis buffer work?
Posted 04 November 2010 - 05:43 AM
I used this recipe:
1 l ddH20
8.26 g Ammonium Chloride (NH4Cl)
1 g Potassium Bicarbonate (KHCO3)
0.037 g EDTA
The rumor has it that it has something to do with the Na+/K+ATPase activity, but this isn't as detailed as I would like to know how it works.
I would very much appreciate an explanation how this buffer work, or maybe a hint to where I can find litterature, or the like, on this.
Posted 11 November 2010 - 11:00 PM
Posted 11 November 2010 - 11:43 PM
Posted 12 November 2010 - 12:08 AM
Well that is also possible I guess. I apologize to all of you nice people who tried to help me out, and I also thank you for your time and effort!