Hi people! I was wondering if somebody could tell me how the red blood cell lysis buffer works. What function do the ingredients have?
I used this recipe:
1 l ddH20
8.26 g Ammonium Chloride (NH4Cl)
1 g Potassium Bicarbonate (KHCO3)
0.037 g EDTA
pH 7.3
The rumor has it that it has something to do with the Na+/K+ATPase activity, but this isn't as detailed as I would like to know how it works.
I would very much appreciate an explanation how this buffer work, or maybe a hint to where I can find litterature, or the like, on this.
Thanks!
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3 replies to this topic
#2
CTC
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Posted 11 November 2010 - 11:00 PM
Come on guys! 80 views and not a single reply! Is my question unclear? Could it be that nobody knows or doesn't any body care to help a fella out?Just a hint on where I could read about it would be very much appreciated as well!
#3
leelee
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Posted 11 November 2010 - 11:43 PM
The other option is that many of these 80 post viewers spent some time looking into this for you (like myself, who spent over an hour on the internet trying to find out) and couldn't find anything more detailed than what you already know..........
#4
CTC
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Posted 12 November 2010 - 12:08 AM
leelee, on 11 November 2010 - 11:43 PM, said:
The other option is that many of these 80 post viewers spent some time looking into this for you (like myself, who spent over an hour on the internet trying to find out) and couldn't find anything more detailed than what you already know..........
Well that is also possible I guess. I apologize to all of you nice people who tried to help me out, and I also thank you for your time and effort!
