5 replies to this topic

[fastaman]

Hi all,

I've been searching the last few days for a software (preferably free) that could draw the arrows that represent ORFs on genes. I have multiple contigs from different bacteria with some ORFs of interest that I would like to draw all together vertically so I can compare these regions between them.

I know I could probably do that on Powerpoint, but I'm sure there is a program somewhere which, given the nucleotide positions of the orfs, could draw them more precisely. I have found one named "FancyGene" (http://host13.bioinf...s.it/fancygene/), but it only draws exons and introns on eukaryotic genes, which is not quite what I need, since the arrows I want to draw are both on the sense and anti-sense strand.

I was expecting something like this picture: http://img688.images...creenshotkc.png



Thanks!
P.

[bob1]

Try ApE (A plasmid editor) which is freeware.

[pDNA]

pDraw32 will also be a  good choice.

You can display your stretch of DNA circular or linear. The programm will find ORFs and you can annotate them ...and color the arrows the way you like it most.

You can finde the programm here.

Regards,
p

[fastaman]

Thank you, guys, I'm installing both of them right now to see which is better.

May the plasmid be with you,
P.

[feilr]

NoeClone also be a good choice
you can display your stretch of DNA circular or linear. The programm will find ORFs and you can annotate them ...and color the arrows the way you like it most. And aslo some other functions.
http://www.noegen.co...oduct_clone.php

[bglick]

SnapGene Viewer (free for Mac and Windows) has a nice modern interface that allows you to display translated features. The program is very easy to use. You can see the features as colored arrows in Map view, and you can see the translations in Sequence view. Translated features can be shown in either direction, and introns can be annotated.

http://www.snapgene....napgene_viewer/