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#1 SciCell

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Posted 02 November 2010 - 12:29 PM

Hello all,

I have a question regarding thawing and using the frozen viral stocks. Some protocols say that we should use the virus immediately after thawing, that is - thaw quickly in a 37 water bath (like frozen cells) etc.. While some protocols say 'don't add cold virus to the cells' so, if I thaw the virus and wait for a few minutes, will the virus be killed? Since it does not have any DMSO, I think virus would not be killed upon thawing. Please let me know different views on this topic.

Thanks

#2 bob1

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Posted 02 November 2010 - 03:06 PM

The not adding cold virus is so as to not temperature shock your cells. If you are only adding a small volume of virus relative to the volume of medium, I wouldn't worry about this effect.

A few minutes at 37 shouldn't kill the virus, though this might depend on the type of virus.




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