Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

pUC19


  • Please log in to reply
1 reply to this topic

#1 MaraJade

MaraJade

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 02 November 2010 - 06:08 AM

Hi,

does pUC19 has a ColE1 Origin which is initiated by Polymerase I? And is the replication goes in direction of the multycloning site or in the other direction?

Thanks in advance and sorry for all those questions ;)

Mara

#2 tfitzwater

tfitzwater

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 96 posts
3
Neutral

Posted 02 November 2010 - 06:41 AM

The primer for replication of pUC initiates near the b-lactamase gene and proceeds towards the Pvu II site near the MCS. It is a ColE 1-type replicon.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.