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Change origin of a plasmid


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4 replies to this topic

#1 MaraJade

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Posted 02 November 2010 - 01:54 AM

Hi,

can I simply change a origin of replication from one plasmid by cutting the old one out and insert a new one from another plasmid?
Can I even change antibiotic resistance or must I regard something special to not disturb the plasmid structure?


Thanks in advance

Mara

#2 phage434

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Posted 02 November 2010 - 03:05 AM

It's all just DNA. Do it.

#3 christy

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Posted 02 November 2010 - 03:58 AM

Changing antibiotic resistance is not a big deal. when u change the origin of replication, take care of the upstream and downstream of the gene,it may interfer in the replication.


Hi,

can I simply change a origin of replication from one plasmid by cutting the old one out and insert a new one from another plasmid?
Can I even change antibiotic resistance or must I regard something special to not disturb the plasmid structure?


Thanks in advance

Mara



#4 MaraJade

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Posted 02 November 2010 - 06:04 AM

Thx

#5 Adrian K

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Posted 02 November 2010 - 08:58 AM

It's all just DNA. Do it.


Wow,
this will be definitely another classical inspirational quote for molecular biologist after Nike's "Just do it".
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




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