I have a Rosa26-loxP-stop-loxP-gfp construct that will be used for making mice overexpressing the gene of interest.
My question is whether I can check functionality of such a plasmid prior to using it in mice. Sequence is OK of course, but how will I know if the gene of interest will be expressed after final transfection and cre recombination?
Do you normally check your constructs by cotransfecting cell lines with the plasmid and a cre expressing plasmid, in order to see if gene expression occurs? or it doesn't work this way?
thanks a lot in advance
Edited by gangut, 01 November 2010 - 05:06 AM.