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Molecular Diagnostic Method by PCR


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#1 PandaCreamPuff

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Posted 31 October 2010 - 01:02 PM

Hi all and happy Halloween. My science life has totally sucked me away from my Halloween night :(

Anyways, I am researching on a PCR-based molecular method for diagnosing etiological bacterial pathogens for mucosal illnesses. One aspect of PCR-based methods is that they do not require viable bacteria, as naked DNA can also be amplified if it's the targeted nucleotide fragment.

So my question is if anyone knows the duration of naked DNA persistence in the mucosal surfaces, specifically the respiratory tract? Because if it persists for a long time, the source of the naked DNA may not be present as an intact cell responsible for illnesses.

Thanks so much.




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