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luciferase's problem or machine's problem?! Does anyone who knows l


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#1 cruz

cruz

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Posted 31 October 2010 - 07:06 AM

Dear all,

I'm doing luciferase assay (pGAS-LUC) for only 3 months, and still couldnt handle it well..really need experts to help me out!! How is the "good" fold difference between negative control(transfeced pgas without ifn-g) and positive control(pgas+ifn-g) in the 293t cell line? Actually I have a lot of questions, but recently I encountered a really confusing one. I usually got >10000 of LCPS, and low (0.1%) LCPS%. However, I got <500 of LCPS, and high (10%)LCPS% in recent two time experiments! What value of LCPS% should be reasonable? I did email the company, but they didnt reply me yet. Our machine is MicroBeta Jet from PerkinElmer. I changed my plasmids and cells, but it still had high LCPS%. Could someone please help me out! Thanks so much. :)

LCPS: Luminescent corrected counts per second divided by 100
LCPS%: Percentage theoretical error of LCPS

Cruz Fan
fanc@ufl.edu




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