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Longer incubation in primary antibody


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#1 cathmet

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Posted 30 October 2010 - 06:50 AM

Hi!
I incubated my blots in primary antibody for more than 24 hrs in 2.5% Milk at 4 degree.
One of my primary is notorious for non specific binding while the others are pretty neat.
Will longer incubation in primary give me a lot of nonspecific binding and background?
Any suggestion will be highly appreciated.

#2 Curtis

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Posted 30 October 2010 - 09:24 AM

No worries, it should be fine. Just wash well with TBST 3x10 min.

Edited by Curtis, 30 October 2010 - 09:24 AM.


#3 cathmet

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Posted 30 October 2010 - 01:03 PM

No worries, it should be fine. Just wash well with TBST 3x10 min.

Thanks!




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