Longer incubation in primary antibody
Posted 30 October 2010 - 06:50 AM
I incubated my blots in primary antibody for more than 24 hrs in 2.5% Milk at 4 degree.
One of my primary is notorious for non specific binding while the others are pretty neat.
Will longer incubation in primary give me a lot of nonspecific binding and background?
Any suggestion will be highly appreciated.
Posted 30 October 2010 - 09:24 AM
Edited by Curtis, 30 October 2010 - 09:24 AM.
Posted 30 October 2010 - 01:03 PM
No worries, it should be fine. Just wash well with TBST 3x10 min.