Hi!
I incubated my blots in primary antibody for more than 24 hrs in 2.5% Milk at 4 degree.
One of my primary is notorious for non specific binding while the others are pretty neat.
Will longer incubation in primary give me a lot of nonspecific binding and background?
Any suggestion will be highly appreciated.
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2 replies to this topic
#2
Curtis
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Posted 30 October 2010 - 09:24 AM
No worries, it should be fine. Just wash well with TBST 3x10 min.
Edited by Curtis, 30 October 2010 - 09:24 AM.
#3
cathmet
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Posted 30 October 2010 - 01:03 PM
Curtis, on 30 October 2010 - 09:24 AM, said:
No worries, it should be fine. Just wash well with TBST 3x10 min.
