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isolate DNA from amniotic fluid


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#1 b612

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Posted 29 October 2010 - 11:32 PM

Hi!!!
I need a method for isolating DNA from amniotic fluid for microarray assay.
I used QIAGEN KIT but i didnīt get enough concentration (<100ng/microL)
Do you Know any good protocol???

#2 Curtis

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Posted 30 October 2010 - 03:11 AM

column based extraction usually gives around that amount. what you got is quite ok. But perhaps you can use phenol/chloroform method next time in case you don't need your sample for qPCR later on. just have a look at protocol-online website. or visit phenol extraction page on wikipedia.

Edited by Curtis, 30 October 2010 - 03:13 AM.





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