Hi!!!
I need a method for isolating DNA from amniotic fluid for microarray assay.
I used QIAGEN KIT but i didnīt get enough concentration (<100ng/microL)
Do you Know any good protocol???
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
Curtis
-
- Global Moderators
-
- 1,112 posts
Metaller Scientist
58
Excellent
Excellent
Posted 30 October 2010 - 03:11 AM
column based extraction usually gives around that amount. what you got is quite ok. But perhaps you can use phenol/chloroform method next time in case you don't need your sample for qPCR later on. just have a look at protocol-online website. or visit phenol extraction page on wikipedia.
Edited by Curtis, 30 October 2010 - 03:13 AM.
